Composite

Part:BBa_K2010006:Design

Designed by: William Cho   Group: iGEM16_Harvard_BioDesign   (2016-06-21)


PETase Composite (T7, His-Tag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 809
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1227


Design Notes

Optimized for E. coli K12. Removed complexities for gBlock ordering.


Source

This part is composite of various parts from various sources. Refer to parts for details.

References